Næringsmiddelteknologi

Komité: CEN/TC 275 (Food analysis - Horizontal methods)
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Sluttdato: 4. okt 2018
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This document describes a screening method for the determination of aflatoxin B1, deoxynivalenol, fumonisin B1 and B2, ochratoxin A, HT-2 and T-2 toxins, and zearalenone in foodstuffs by high performance liquid chromatography (HPLC) coupled with tandem mass spectrometry (MS/MS). The aim of the screening method is to test compliance of foodstuff with regulatory limits or to determine whether a certain pre-defined level (the screening target concentration, STC) is exceeded or not. The result of the screening is either "negative" or "suspect". "Negative" (screen negative) means that the targeted mycotoxins are not detected or potentially present but below the STC. "Suspect" (screen positive) means that the established cut-off level is exceeded and the sample can contain one or more mycotoxins at a level higher than the STC. For full identification and accurate quantification a second confirmatory quantitative analysis method is required which is outside the scope of this document. The method is suitable for various types of foodstuff and has been validated for representative matrices from four commodity groups: - high starch and/or protein content and low water and fat content: wheat, cereal mixture, wheat flour and cornflakes; - high oil content: peanuts; - high sugar low water content: figs; - high water content: grape juice. During validation, cut-off levels were established for the following screening target concentrations: - aflatoxin B1: 2 µg/kg to 5 µg/kg; - deoxynivalenol: 250 µg/kg to 865 µg/kg; - fumonisin B1: 200 µg/kg to 790 µg/kg; - fumonisin B2: 110 µg/kg to 230 µg/kg; - ochratoxin A: 4 µg/kg to 9 µg/kg; - T-2 toxin: 25 µg/kg; - HT-2 toxin: 25 µg/kg to 50 µg/kg; - zearalenone: 30 µg/kg to 100 µg/kg.
Komité: CEN/TC 275 (Food analysis - Horizontal methods)
Opprinnelse:
Sluttdato: 4. okt 2018
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This document describes a procedure for the determination of nivalenol (NIV), deoxynivalenol (DON) and its acetyl derivatives (3-acetyl-DON and 15-acetyl-DON), HT-2 and T-2 toxins (HT-2, T-2) and zearalenone (ZEA) in cereals and cereal products by high performance liquid chromatography (HPLC) coupled with tandem mass spectrometry (MS/MS) after cleanup by solid phase extraction (SPE). The method has been validated with both contaminated and spiked samples of wheat, wheat flour, and wheat crackers. Validation levels for NIV ranged from 27,7μg/kg to 377,8 μg/kg. Validation levels for DON ranged from 233,9μg/kg to 2420,0 μg/kg. Validation levels for 3-acetyl-DON ranged from 18,5μg/kg to 136,5 μg/kg. Validation levels for 15-acetyl-DON ranged from 11,4μg/kg to 141,8 μg/kg. Validation levels for HT-2 ranged from 6,6 μg/kg to 133,8 μg/kg. Validation levels for T-2 ranged from 2,1 μg/kg to 37,6 μg/kg. Validation levels for ZEA ranged from 31,6μg/kg to 229,7 μg/kg Laboratory experiences have shown that this method is also applicable to barley and oat flour, and rye based crackers [5], however, this has not been validated in a collaborative study.
Komité: CEN/TC 307 (Oilseeds, vegetable and animal fats and oils and their by-products - Methods of sampling and analysis)
Opprinnelse:
Sluttdato: 30. okt 2018
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Komité: CEN/TC 307 (Oilseeds, vegetable and animal fats and oils and their by-products - Methods of sampling and analysis)
Opprinnelse:
Sluttdato: 15. nov 2018
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The purpose of this document is to describe a procedure for the determination of the ester content in fatty acid methyl esters (FAME) intended for incorporation into diesel oil. It also allows determining the linolenic acid methyl ester content. It allows verifying that the ester content of FAME is greater than 90 % (m/m) and that the linolenic acid content is between 1 % (m/m) and 15 % (m/m). This method is suitable for FAME which contains methyl esters between C6 and C24. NOTE 1 For the purposes of this document, the terms "% (m/m)" and "% (v/v)" are used to represent respectively the mass and volume fractions. NOTE 2 This method was elaborated for FAME samples from usual raw material. For FAME sample from unidentified raw material, a solution of the test sample should be prepared without any internal standard addition, in order to verify the absence of natural nonadecanoic acid methyl ester. NOTE 3 The distribution off fatty acid methyl esters is given in Annex C. WARNING - The use of this method may involve hazardous equipment, materials and operations. This method does not purport to address to all of the safety problems associated with its use, but it is the responsibility of the user to search and establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.