Dyrefor

This document specifies a high performance liquid chromatographic – tandem mass spectrometry (LC-MS/MS) method for the simultaneous screening and/or determination of the eleven authorised coccidiostats (halofuginone, robenidine hydrochloride, nicarbazin, diclazuril, decoquinate, monensin sodium, salinomycin sodium, narasin, lasalocid sodium, semduramicin sodium and maduramicin ammonium alpha) contents in poultry, cattle and pig feed at additive and cross-contamination levels and of five non-registered coccidiostats (ethopabate, clopidol, ronidazole, dimetridazole and amprolium) at sub-additive levels and for the screening of the prohibited furazolidone antibiotic at sub-additive level, in the same matrices. The range of application of the method is fit for the purpose of the screening and determination of all eleven coccidiostats at the values set by European legislation, of the non-registered coccidiostats and of the screening of the banned antibiotic.

This document specifies a method for the determination of organic acids in animal feeding stuffs by Ion Chromatography with conductivity detection (IC-CD). The method is intended to be used for the determination of formic acid, lactic acid, propionic acid, citric acid, fumaric acid and malic acid as active substances in feed additives, premixtures, compound feed and water and for screening of acetic acid in the same matrices. This method determines the total extractable concentration of the above mentioned organic acids and their salts. The working range of the method must be determined for each organic acid by the user of this standard. The lower limit of the working range depends on the matrix and the interferences encountered. A working range between 10 [mg/l] and 100 [mg/l] should be achievable. The method was successfully tested in an inter-laboratory study in concentrations between 0,02 % up to 27 % of the above mentioned organic acids. NOTE Limitation occurs during simultaneous determination of high concentration of lactic acid and low concentration of acetic acid. If the ratio of concentration of lactic acid to acetic acid exceeds factor 20, the determination of acetic acid is not guaranteed. On the basis of the referred working range, sample weight and extraction volume, limits of quantification (LOQ), as calculated (Table 1) should be achievable.

This document specifies a method for the determination of benzoic acid and sorbic acid in animal feeding stuffs by high-performance liquid chromatography method with ultra-violet detection (HPLC-UV). The method is intended to be used for the determination of benzoic acid and sorbic acid as active substances in feed additives, premixtures and compound feed and for benzoic acid in water. This method determines the total extractable concentration of these organic acids and their salts. The working range of the method must be determined for each organic acid by the user of this standard. The lower limit of the working range depends on the matrix and the interferences encountered. A working range between 5 mg/l and 100 mg/l should be accessible. The method was successfully tested in an inter-laboratory study in concentrations between 0,02 % up to 9,0 %. On the basis of the referred working range, sample weigh and extraction volume, limits of quantification (LOQ), as calculated (Table 1) on the basis of a wavelength of 230 nm, should be achievable.